Modulation of Antisense Phosphorothioate Oligodeoxynucleotides of Epidermal Growth Factor Receptor on E-cadherin mRNA Expression in Human Hepatocellular Cell
10.3321/j.issn:1000-467X.2001.03.006
- VernacularTitle:EGFR反义寡脱氧核苷酸硫代修饰片段调节人肝癌细胞中E-cadherin mRNA表达的研究
- Author:
Yan-Jing GAO
1
;
Meng-Biao YUAN
;
XIN-Hua
;
WANG-Qian
;
Hong-Lian SHAO
Author Information
1. Qilu Hospital Shandong University
- From:Chinese Journal of Cancer
2001;20(3):255-257
- CountryChina
- Language:Chinese
-
Abstract:
Objectives：This study was designed to investigate modulation of phosphorothioate oligodeoxynucleotides(S-ODNs) of EGFR on E-cadherin mRNA expression in human hepatocellular carcinoma cells. Methods：Treated with concentration of 3.2 μ mol/L S-ODNs in culture medium， the relative E-cadherin mRNA level at 84 hour in human hepatocellular carcinoma cells was evaluated by reverse-transcriptase polymerase chain reaction with using HPRT (hypoxanthine phosphoribosyltransferase) as an internal control standard. Result：The expression of E-cadherin gene mRNA was enhanced obviously by S-ODNs. Compared with control group, the relative expression level of E-cadherin gene mRNA was increased at about 66.36％ (P<0.05). Conclusion： S-ODNs is the regulator of E-cadherin gene expression, it can enhanced E-cadherin gene expression in human hepatoma cells. Understanding the regulation of E-cadherin gene expression in human hepatocellular carcinoma cells might contribute to development of a new preventive and therapeutic strategy for tumor invasiveness.
