Primary construction and application of National bank for HIV-1 standard strains
10.3760/cma.j.cn112866-20230504-00046
- VernacularTitle:HIV-1标准株资源库的初步建立与应用
- Author:
Li REN
1
;
Lin HE
;
Meiling ZHU
;
Yan WANG
;
Desheng KONG
;
Chen WANG
;
Yi FENG
;
Yiming SHAO
;
Hui XING
;
Chuntao MA
;
Liying MA
Author Information
1. 中国疾病预防控制中心性病艾滋病预防控制中心，北京 102206
- Keywords:
Human immunodeficiency virus;
National standard isolation;
Virus amplification;
Resource Library;
Application
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(3):280-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct standard strains representing the main epidemic clades of HIV-1 in China, amplify the virus strains, and establish a seed lot.Methods:Six isolates of HIV-1 virus were identified and analyzed in genotype and phenotype, according to " interpretation for the social organization of the Standard strains of pathogenic microorganism- technical specifications for establishment of HIV strains". The isolates were amplified and cultivated to generate the secondary generation primary seed lot and the third generation working seed lot as frozen storage in liquid nitrogen. Results:Six HIV-1 standard strains were obtained, of which 3 strains are CRF_ 07BC (NRPC2.4.9003, NRPC2.4.9005, NRPC2.4.9006), 1 strain is CRF_ 01AE (NRPC2.4.9001), 1 strain is CRF_ 08BC (NRPC2.4.9002), and 1 strain is URF (NRPC2.4.9004). Phenotypic detection showed that all six strains are CCR5 tropics and Non syncytia inducing virus. TCID 50 were all greater than 1 × 10 5/ml, and concentrations of p24 antigen were all higher than 2 ng/ml. A primary seed lot with no less than 20 vials per strain and a working seed lot with no less than 50 vials per strain were constructed. The standard virus strains were used in evaluating antiviral drugs PEG2kC34, PEG5kC34, LP-19, and neutralizing antibody LSEVh LS-F. Conclusions:Six standard strains of HIV-1 virus covering the three main epidemic subtypes of HIV-1 in China have been obtained, and a storage of HIV-1 standard strain was constructed. It meets the need of the preservation of HIV-1 standard strains in China and provides support for drug and vaccine evaluation.
