Expression of STAT3 and P-STAT3 in the brain of a transgenic mouse model of Alzheimer's disease
10.3969/j.issn.1673-4254.2013.12.15
- VernacularTitle:STAT3与P-STAT3在转基因AD小鼠脑组织中的表达及意义
- Author:
Mi TIAN
1
;
Deren HOU
;
Yanyao DENG
;
Wei LI
;
Xialu FENG
Author Information
1. 中南大学湘雅三医院神经内科
- Keywords:
STAT3;
P-STAT3;
transgenic mice;
immunohistochemistry;
Alzheimer's disease
- From:
Journal of Southern Medical University
2013;(12):1778-1782
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of signal transducer and activator of transcription 3 (STAT3) and P-STAT3 in the brain of the APPswe/PS△E9 double transgenic mouse model of Alzhaimer's disease (AD) and invesitgate their possible role in AD. Methods APPswe/PS △E9 double transgenic mice and control mice were examined for cerebral STAT3 and P-STAT3 expressions using immunothistochemistry. Results STAT3 and P-STAT3 were expressed in the different regions of mouse brain. In the transgenic mice and the control mice, the positivity rates of STAT3 were 93.75%and 87.50%in the cerebral cortex, 87.50% and 43.75% in the basal forebrain, 81.25% and 37.50% in the hippocampus, and 62.50% and 0.00% in the cerebellum, respectively, showing significant differences between the mice in the STAT3 expressions in the basal forebrain, hippocampus and cerebellum (P<0.05). The positivity rates of P-STAT3 in the two groups were 0.00%and 0.00%in the cerebral cortex, 68.75%and 0.00% in the basal forebrain, 62.50% and 12.50% in the hippocampus, and 43.75% and 0.00% in the cerebellum, respectively, showing also significant differences in the basal forebrain, hippocampus and cerebellum (P<0.05). The expression of STAT3 was positively correlated with that of P-STAT3 in transgenic AD mice (P<0.05). Conclusion STAT3 and P-STAT3 are highly expressed in the basal forebrain, hippocampus and cerebellum in transgenic AD mice and may participate in the pathological process of AD.
