Expression of Telomerase Subunits in Nasopharyngeal Carcinoma
10.3321/j.issn:1000-467X.2001.03.004
- VernacularTitle:鼻咽癌组织端粒酶各组分基因表达的研究
- Author:
Xing-Wei WANG
1
;
Jian-Yun XIAO
;
Su-Ping ZHAO
;
Yong-Quan TIAN
;
Guang-Ping WANG
Author Information
1. 湖南医科大学附属湘雅医院
- From:Chinese Journal of Cancer
2001;20(3):245-248
- CountryChina
- Language:Chinese
-
Abstract:
Objective：This study was designed to ascertain telomerase subunits expression in nasopharyngeal carcinoma (NPC) as well as in chronic inflammation of nasopharyngeal epithelium (CINE). Methods：Telomerase subunits(hTR,TP1 mRNA, hTERT mRNA) expression was determined with reverse transcription-polymerase chain reaction (RT-PCR) in 43 cases of NPC and 16 cases of CINE. Results：The positive rates of hTR,TP1 mRNA and hTERT mRNA were 90.7％ ,88％ , and 88％ in 43 NPCs, and 87.5％ , 87.5％ , and 0 in 16 CINEs respectively. hTERT mRNA exoressuib was significantly higher in NPC than in CINE,but no statistical difference for hTR or TP1 mRNA occurred in two groups of patients. Conclusion：These findings revealed that hTR and TP1 mRNA were widely expressed in both NPC and CINE, but hTERT mRNA was limited in NPC, which suggests that hTERT mRNA may play a critical role in activation of telomerase. The authers’findings also indicate that detection of hTERT mRNA may be a useful maker for clinical diagnosis of NPC.
