Laboratory identification and evaluation of national standard strains of Japanese encephalitis virus G1/G3/G5
10.3760/cma.j.cn112866-20230522-00054
- VernacularTitle:G1/G3/G5型乙型脑炎病毒国家标准株实验室鉴定与评价
- Author:
Shenghui LIU
1
;
Mengnan JIANG
;
Weijia ZHANG
;
Shihong FU
;
Jingdong SONG
;
Chongxiao XU
;
Kai NIE
;
Qikai YIN
;
Ying HE
;
Fan LI
;
Songtao XU
;
Guodong LIANG
;
Qiang WEI
;
Huanyu WANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病预防控制国家重点实验室，北京 102206
- Keywords:
Japanese encephalitis virus;
National standard strains;
identification and evaluation
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(3):273-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the evaluation indexes of national standard strains of genotypes 1, 3 and 5 of Japanese encephalitis virus (JEV) and evaluate the national standard JEV strains.Methods:According to the national standard strain evaluation technical standards of pathogenic microbial bacteria (virus) species, based on the application of Japanese encephalitis virus research, and according to the morphological characteristics, biological characteristics, molecular biological characteristics and other research data to identify the characteristics of G1, G3 and G5 genotypes of JEV.Results:Spherical virus particles with a diameter of about 60 nm were visible under electron microscope of the three Japanese encephalitis virus strains. The cytopathic effect was mainly characterized by cell shrinkage and exfoliation in BHK-21 and Vero cell lines, cell fusion and exfoliation were shown after infection with C6/36 cell line; the virus titer was 10 5-10 7 PFU/ml, and the plaque size was different by genotype. The median lethal dose of intrabitoneal challenge in G1, G3 and G5 JEV in three weeks-old mice was 50.51 PFU, 6.98 PFU, and 8.13 PFU, and the median lethal dose of intracranial challenge in five weeks mice was 3 PFU, 0.3 PFU, 1.35 PFU. The whole genome length of G1, G3 and G5 JEV was 10 967 bp, 10 976 bp and 10 983 bp, respectively. Conclusions:Three genotypic national standard strains of JE V were identified and evaluated by electron microscopy, cell, animal and genome laboratory indexes, which provided reference for the identification and evaluation of other national standard strains of JEV.
