Correlation of Apoptosis and N-myc Gene Expression in Neuroblastoma Induced by Arsenic Trioxide and Homoharringtonine
10.3321/j.issn:1000-467X.2001.03.003
- VernacularTitle:三氧化二砷和高三尖杉诱导神经母细胞瘤细胞凋亡与N-myc表达的关系
- Author:
Qin XU
1
;
Jing-Yan TANG
;
Gang WU
;
Wen-Zhu ZHANG
;
Zi LIN
;
Tong-Xin CHEN
;
Da-Ming YING
Author Information
1. Xinhua Hospital Shanghai Institute for Pediatric Research Shanghai Second Medical University
- From:Chinese Journal of Cancer
2001;20(3):239-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective：This study was designed to explore the mechanism of inhibition of proliferation induced by arsenic trioxide (As2O3) and homoharringtonine in neuroblastoma. Method：Methy thiazoloyl tetrazolium (MTT) methods and analysis of apoptosis were used to evaluate the effect of As2O3 and homoharringtonine on the proliferation of NB cell lines and their mechanisms. Reverse-transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry techniques were used in NB cell lines treated with As2O3 and HHT to determine N-myc mRNA and protein expression. Results：Both As2O3 and HHT can significantly inhibit the proliferation of neuroblastoma cell lines and induce apoptosis of SJ-N-SH and IMR-32 cells. In As2O3 treated cell line, at transcription and protein level, N-myc expression is up-regulated at first and then down-regulated to a lower level. HHT can down-regulate N-myc protein. Conclusion：N-myc may play some roles in apoptosis induced by As2O3 and HHT, but the mechanisms initiating N-myc gene may be different.
