Detection of Salivary Epstein-Barr Virus Antibodies for Early Diagnosis of Nasopharyngeal Carcinoma
10.3321/j.issn:1000-467X.2001.03.002
- VernacularTitle:唾液中EB病毒抗体检出用于早诊鼻咽癌的研究
- Author:
Yi-Xin ZHENG
1
;
Ji-Zhong LI
;
Shao-Wen JIAN
;
Duan LI
;
Man-Zhi LI
;
Li-Bing SONG
;
Ling ZHANG
;
Hui-Min WANG
Author Information
1. Sun Yat-sen University of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(3):235-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective：This study was designed to establish a salivary EBV-EA IgA and DNase IgA test technique, and seek a fast and specific diagnostic technique for nasopharyngeal carcinoma (NPC). Methods：Polypeptides of EBV-DNase(ED) and EA-D was synthesized as catching antigens. With ELISA technique, IgA/ED and IgA/EA-D were evaluated respectively in saliva and serum from NPC patients and healthy volunteers. Results：After statistic analysis of the optical density(OD) values of samples, the diagnostic criteria of NPC in the examination of either IgA/ED or IgA/EA-D was defined as following：OD≥ 0.3 for serum and OD≥ 0.45 for saliva. Significantly statistical difference existed between the values of either IgA/ED or IgA/EA-D titer in patients with NPC and the values in healthy volunteers,P<0.0001. The coincidence rates between the diagnosis of above IgA/ED or IgA/EA-D titer testes and corresponding histological diagnosis were 72.6％ － 77.3％ . Conclusion： The Elisa test to detect salivary IgA/ED and IgA/EA-D with synthesized polypeptides is a simple, repeatable, and cheap technique with stability and sensitivity. However its coincidence rate with histology should be improved.?
