Large-Scale Screening for Genes Related to Pathogenesis of Pituitary Carcinoma
10.3321/j.issn:1000-467X.2001.03.001
- VernacularTitle:垂体瘤发病相关基因的大规模筛选及鉴定
- Author:
Huai-Dong SONG
1
;
Ren-Ming HU
;
Jia-Lun CHEN
;
Yong-De PENG
;
Qiu-Hua HUANG
;
Juan ZHOU
;
Xin-Yan WU
;
Qing-Hua ZHANG
;
Shuang-Xi REN
;
Gang FU
Author Information
1. Ruijin Hospital Shanghai Institute of Endocrinology Shanghai Institute of Hematology Shanghai Second Medical University
- From:Chinese Journal of Cancer
2001;20(3):229-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The aim of this study was to investigate the method of large-scale screening for genes differentially expressed in nonfunctional pituitary tumor and normal pituitary. Methods： cDNA libraries of nonfunctional pituitary tumor (NFPT) and human normal pituitary were constructed. Large-scale sequencing, bioinformatics and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) were used. Significant difference of known genes expression in 2 tissues were analyzed by statistical software offered by Audic S and Claverie JM (http： //igs-server.cnrs-mrs.fr/). Results： A total of 1253 and 7222 ESTs with good quality were obtained from the tissues of NFPT and normal pituitary, respectively. Two hundred known genes were found in NFPT. Among them, 38 genes creditability of significant difference expression in the 2 tissues were over 0.99, and 130 were over 0.95. Out of the 130 differentially expressed genes in the 2 tissues, 17 genes were associated with cell differentiation and development (G2). 6 differentially expressed G2 category genes were selected and the liability of the expression significant difference in the 2 tissues was confirmed by semi-quantitative RT-PCR. Out of them, 4 were confirmed differentially expressed in the 2 tissues. Conclusion： MEIS2, SMT3C, C1D, and BUB3 genes associated with cell differentiation and development (G2) were expressed more in nonfunctional pituitary tumor than in normal pituitary. They may play a role in the pathogenesis of nonfunctional pituitary carcinoma.
