Research progress of the SARS-CoV-2 nucleic acid diagnostic method based on CRISPR system
10.3760/cma.j.cn112866-20220719-00162
- VernacularTitle:基于CRISPR系统的新型冠状病毒核酸诊断方法研究进展
- Author:
Mengwei NIU
1
;
Hao LI
;
Rui XIAO
;
Yao HAN
;
Yansong SUN
Author Information
1. 军事科学院军事医学研究院微生物流行病研究所，北京 650500
- Keywords:
CRISPR/Cas system;
SARS-CoV-2;
nucleic acid detection technology;
SARS-CoV-2 variants
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(2):227-236
- CountryChina
- Language:Chinese
-
Abstract:
The coronavirus disease 2019 (COVID-19) epidemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still ongoing. With the continuation of the epidemic, the SARS-CoV-2 genome is constantly mutating and evolving, producing more and more SARS-CoV-2 variants, which has brought severe pressure to the prevention and control of COVID-19. In view of the spread of COVID-19, it is extremely important to confirm the infection with SARS-CoV-2 and identify SARS-CoV-2 variants through nucleic acid detection. Therefore, it is urgent to develop highly sensitive and highly specific detection method for different application scenes, such as on-site, high-throughput and automated detection, to realize the diagnosis of SARS-CoV-2 and its variants. At present, researchers have used clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection technology combined with lateral flow strips and other technologies to establish a variety of diagnostic method for SARS-CoV-2 and SARS-CoV-2 variants, which can be applied to different scenes and regions. In this article, we summarize the research progress of CRISPR-based SARS-CoV-2 diagnostic method.
