Application of digital PCR in detection of gastrointestinal viruses
10.3760/cma.j.cn112866-20221225-00256
- VernacularTitle:数字PCR技术在胃肠道病毒检测中的应用进展
- Author:
Yu WANG
1
;
Wen BAO
;
Jiaxuan BAI
;
Xiaoru CHAI
;
Juntao MENG
;
Shiyou LIU
;
Cong JIN
;
Xiangdong XU
Author Information
1. 河北省环境与人群健康重点实验室 河北医科大学公共卫生学院，石家庄 050017
- Keywords:
Digital PCR;
Gastrointestinal virus;
Detection technology
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(2):222-226
- CountryChina
- Language:Chinese
-
Abstract:
Gastrointestinal viruses include acute gastroenteritis virus and enterovirus. These viruses are highly contagious and human populations are generally susceptible to them, and the viruses require only tens to hundreds of virus particles to cause infection. Digital polymerase chain reaction (dPCR) has the advantages of high sensitivity, strong anti-interference and direct quantification. It has shown its uniqueness in the detection of gastrointestinal viruses, especially for samples with low viral loads, which is a beneficial supplement to the real-time PCR technology. This article reviews and looks forward to the application of digital PCR technology in gastrointestinal virus detection.
