Curcumin inhibits HeLa cell invasion and migration by decreasing inducible nitric oxide synthase
10.3969/j.issn.1673-4254.2013.12.09
- VernacularTitle:姜黄素通过降低一氧化氮合酶水平抑制宫颈癌HeLa细胞的侵袭和转移
- Author:
Mu LI
1
;
Li WANG
;
Haili LIU
;
Baoshan SU
;
Bianli LIU
;
Wenjing LIN
;
Zhaorong LI
;
Lihua CHANG
Author Information
1. 西安交通大学医学院第二附属医院妇产科
- Keywords:
curcumin;
cervical cancer;
invasion;
metastasis;
HeLa cells;
nitric oxide synthase
- From:
Journal of Southern Medical University
2013;(12):1752-1756
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of curcumin against HeLa cell invasion and migration and explore the underlying mechanisms. Methods HeLa cells were exposed to curcumin treatment at the concentrations of 0, 10, 25, 50, 100, 150 and 200 μmol/L for 24 h. MTT and TUNEL assays were used to assess the cell proliferation inhibition and apoptosis, respectively. Transwell assay was used to evaluate the invasiveness and migration of the treated cells, and RT-PCR and Western blotting were employed to detect the changes in the expression of inducible nitric oxide synthase (iNOS), and MMP-9 and E-cad, the 2 markers of cell invasion and migration, were detected by Western blotting. The capacity of NO production in HeLa cells was measured by Griess method. Results Curcumin inhibited the proliferation of HeLa cells by inducing cell apoptosis in a concentration-dependent manner. Curcumin inhibited the invasion and migration of HeLa cells by increasing E-cad expression and decreasing MMP-9 expression, and also decreased the expression level of iNOS and NO production in the cells. Conclusions Curcumin inhibits the invasion and migration of HeLa cells by decreasing the expression of iNOS.
