Analysis of genetic evolution of parechovirus in neonates from Hunan Provincial People’s Hospital in 2021
10.3760/cma.j.cn112866-20220802-00171
- VernacularTitle:2021年湖南省人民医院新生儿副肠孤病毒的遗传进化分析
- Author:
Xiaohua MA
1
;
Leyun XIE
;
Sasa CHAI
;
Shenghui GAO
;
Aimin ZHANG
;
Tian YU
;
Guisen ZHENG
;
Lili LI
;
Saizhen ZENG
;
Zhaojun DUAN
Author Information
1. 甘肃中医药大学公共卫生学院，兰州 730000
- Keywords:
Neonate;
Parechovirus;
Genetic evolution analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(2):184-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the prevalence of human parechovirus (HPeV) in neonates of Hunan Provincial People’s Hospital, and analyze genetic evolutionary characteristics.Methods:From June to September 2021, fecal samples of inpatient neonates were collected in Hunan Provincial People′s Hospital. TaqMan real-time qPCR and RT-PCR were used for HPeV screening and genotyping. High-throughput sequencing and PCR were used to obtain whole genomes. Phylogenetic analysis was performed after sequencing.Results:A total of 123 fecal samples of neonates were collected, of which 22 were HPeV positive, with 17.89% positive rate. All the strains belonged to the HPeV-1 genotype. One full-length genomic sequence of 7 269 bp were obtained, and provisionally named Hunan/HPeV/2021, which has the highest nucleotide identity with known HPeV-1 genotype, with 86.6%-91.9% nucleotide identity. The nucleotide and amino acid identity of open reading frame (ORF) with known similar sequences were 90.3%-92.6% and 97.3%-98.3%, respectively. The phylogenetic analysis showed that Hunan/HPeV/2021 belongs to the HPeV-1 genotype, which is clustered into the same clade as the popular HPeV-1 strains in China.Conclusions:HPeV has a high prevalence in inpatient neonates of Hunan Provincial People’s Hospital and belong to the HPeV-1 genotype.
