Expression of bcl-2 in Lung Cancer:a Mechanism for Apoptosis Antagonism and Immune Evasion
10.3321/j.issn:1000-467X.2001.02.012
- VernacularTitle:bcl-2基因表达与肺癌细胞凋亡的关系
- Author:
De-Kun WANG
1
;
Jun-Meng ZHENG
;
Hai-Ming JIANG
;
Zhu-Lin ZHANG
;
Jing-Fang ZHANG
;
Neng-Tai OUYANG
Author Information
1. Cancer Institute of Zhongshan
- From:Chinese Journal of Cancer
2001;20(2):168-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The current study was designed to investigate the relationship between bcl-2 expression and apoptosis of lung cancer cells in vitro. Methods:Lung cancer cell lines with different expression of bcl-2 were cocultured with tumor infiltrating lymphocyte(TIL) isolated from fresh tumor samples,and JAM test was performed to evaluate apoptosis of cancer cells. Immunohistochemistry and TUNEL assay were used to determine bcl-2 expression and apoptosis of tissue sections of lung cancers respectively. Results: As shown in JAM test, apoptosis increased with the elevation of TIL in coculturing assay in bcl-2 negative cancer cell lines, but in one of bcl-2 positive cell lines it remained stable. Conclusions: Lung cancer cells with bcl-2 expression may antagonize apoptosis, which may account for their immune evasion mechanism.
