Mechanism of "Sanyang" combined therapy of traditional Chinese medicine in alleviating colonic injury in mice induced by influenza virus based on transcriptome sequencing technique
10.3760/cma.j.cn112866-20221117-00236
- VernacularTitle:基于转录组测序技术分析中医三阳合治法减轻流感病毒致小鼠结肠损伤的作用机制
- Author:
Yanan ZHANG
1
;
Jun YAN
;
Liqiong SONG
;
Yuanming HUANG
;
Chang LIU
;
Guoxing LIU
;
Jintong LI
;
Yue ZHANG
;
Mingzhe WANG
;
Zhiguang ZHAI
;
Chengxiang WANG
;
Lishan ZHANG
;
Chengjun BAN
;
Wenbo XU
;
Miao CHENG
Author Information
1. 北京中医药大学东直门医院，北京 100700
- Keywords:
Ma-Xing-Shi-Gan Decoction combined with Xiao-Chai-Hu Decoction;
Sanyang combined therapy (SCT);
Influenza virus;
Transcriptome sequencing;
Colon injury;
Me
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(2):159-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Ma-Xing Shi-Gan decoction combined with Xiao-Chai-Hu decoction (hereinafter referred to as " Sanyang combined treatment" ) in alleviating colon injury in mice infected with influenza virus by transcriptome sequencing technique.Methods:The mouse model of colonic injury caused by influenza virus was induced by intranasal drip of influenza A virus H1N1 suspension. The mice were divided into Control group, Model group, and Sanyang combined treatment (SCT) group. Model group and SCT group were fed with PBS and Ma-Xing Shi-Gan decoction combined with Xiao-Chai-Hu decoction respectively. Seven days later, the colon tissues of each group were taken, the colon length and pathological damage were observed, and the transcriptome was sequenced to screen the significantly different genes between the SCT group and model group for Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis(GSEA).Results:After the therapy with SCT, the length of the colon of mice was significantly improved and the pathological injury of the colon was reduced. There are 92 differentially expressed genes between the SCT group and the model group. GO analysis indicated that the differential genes were enriched in biological processes such as regulation of cytokine and chemokine production, inflammatory response, defense response, immune response, regulation of NF-κB inducing kinase(NIK)/Nuclear factor-κB(NF-κB) signal and Mitogen-activated protein kinase(MAPK) cascade, as well as cell components related to intestinal barrier such as brush border membrane, brush border and microvilli. KEGG analysis indicated that the differential genes were enriched in Toll-like receptor signaling pathway, intestinal immune network for IgA production, complement and coagulation cascade, and Peroxisome proliferator-activated receptor(PPAR) signaling pathway. GSEA indicated that the intestinal immune network for IgA production, PPAR signaling pathway, propionic acid metabolism and butyrate metabolism were significantly up-regulated after the intervention with SCT, while apoptosis and MAPK signaling pathway were significantly down-regulated.Conclusions:Sanyang combined therapy can protect the intestinal tract of mice infected with influenza virus mainly through immunity, inflammation and metabolism pathways.
