Biological characteristics in vitro and mouse toxicities differences among three attenuated poxvirus vaccine candidates
10.3760/cma.j.cn112866-20230208-00010
- VernacularTitle:3个非复制型痘苗病毒基因修饰株细胞生物学特性及小鼠体内毒力比较
- Author:
Yamei DOU
1
;
Jiao REN
;
Li ZHAO
;
Hang YUAN
;
Xin MENG
;
Houwen TIAN
;
Wenling WANG
;
Wenjie TAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒与病毒病重点实验室，北京 102206
- Keywords:
Vaccinia virus;
Replication defective;
Smallpox/Monkeypox;
Virulence;
Vaccine
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(2):146-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Through the study of the cell biological characteristics and virulence in mice in vivo of three non-replicating vaccinia virus modified strains to provide reference for the development of smallpox/monkeypox vaccine replacement products.Methods:Replicating vaccinia virus Tiantan strain VTT and non-replicating Tiantan Vaccinia Strain NTV were studied in BHK-21/CEF and its modified strains NTV-C7L, NTV-△F1L-C7L, NTV-K1L were amplified, purified, and identified by Western blotting. The virulence and diffusivity of each strain in cells were evaluated by immune-plaque assay. The replication dynamics curves were used to compare the replication differences between the strains, and the weight loss was observed by intranasal route in the mouse model to compare the virulence levels of the viruses.Results:In this study, Western blotting result proved that the amplified and purified vaccinia virus strains were correct. Immunophagocytosis and replication kinetics showed that the replication capacity of the three NTV modified strains in CEF was similar to that of NTV. The diffusion ability and replication ability between Vero cells were improved, but the replication multiple was less than 100 times. The replication level of MRC-5 was significantly enhanced compared with that of NTV, and the replication ratio of NTV-C7L was more than 20000 times. The virulence in mice showed that the body weight of the three NTV modified strains had no statistical significance compared with that of NTV.Conclusions:The three NTV modified strains recovered their replication ability in human MRC-5 cells, but their virulence in mice was similar to that of NTV, which provided the preliminary conditions for being candidate strains of smallpox/monkeypox vaccine replacement products.
