Relationship between the Pattern of Methylation of Calcitonin Gene and Activity of Methyltransferase in 8 Tumor Cell Lines
10.3321/j.issn:1000-467X.2001.02.009
- VernacularTitle:8种肿瘤细胞系降钙素基因甲基化模式及其与甲基转移酶活性的关系
- Author:
Xiao-Chuan BAI
1
;
Zhi-Yong BAI
;
Shu-Lan WU
Author Information
1. the First Teaching Hospital Beijing Medical University
- From:Chinese Journal of Cancer
2001;20(2):156-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The current study was designed to investigate the relationship between the pattern of methylation of calcitonin (CT) gene and the activity of DNA methyltransferase (MTase). Methods: The methylation rate of 5′ region of CT gene (CTMR) and the activity of MTase in 6 solid tumor cell lines and 2 leukemia cell lines were determined. CTMR was detected by polymerase chain reaction(PCR) with inter and external references in combination with restriction endonuclease and laser scan technique. MTase activity was examined by isotope labeled microassay. Results:Both CTMR and MTase activity in all tumor cell lines were significantly higher than that of control cells. The increased MTase activity was relative to elevated CTMR. Conclusion: There is prevalence of hypermethylation of CT gene and elevated activity of MTase in malignant cells. The increased MTase activity is one of the possible reasons for CT gene hypermethylation in tumor cells.
