Hepatopoietin Is an Autocrine Stimulator for Autonomous Growth in Hepatoma Cell Line
10.3321/j.issn:1000-467X.2001.02.007
- VernacularTitle:肝细胞生成素通过自分泌循环刺激肝癌细胞系的增殖
- Author:
Yong LI
1
;
Gui-Chun XING
;
Qing-Ming WANG
;
Ji-Zhong CHEN
;
Hui-Peng CHEN
;
Fu-Chu HE
Author Information
1. Institute of Radiation Medicine
- From:Chinese Journal of Cancer
2001;20(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current study was designed to clarify whether hepatopoietin (HPO) stimulates autonomous growth of hepatoma cell by autocrine loop. Methods： The authors conducted experiments in vitro with hepatoma cell lines. RT-PCR, ELISA and Western blot were used to examine HPO expression in hepatoma cells. Blocking effect of HPO by HPO neutralizing antibody was utilized and the changes of cell proliferation was observed. Results： HPO was expressed by hepatoma cells and secreted into the medium. Moreover, the HPO antibody inhibited specifically the autonomous proliferation of hepatoma cell and antagonized the stimulatory effect of concentrated conditioned medium derived from hepatoma cell HepG2. Conclusion： The results strongly suggest that HPO acts as an autocrine factor to maintain the autonomous growth of hepatoma cells.
