Imbalance of Th17/Treg induced by coxsackievirus B3 leads to viral pancreatitis in mice
10.3760/cma.j.cn112866-20230209-00013
- VernacularTitle:CVB3诱导Th17/Treg失衡导致小鼠病毒性胰腺炎
- Author:
Ruifang WANG
1
;
Kexin ZONG
;
Qinqin SONG
;
Dong XIA
;
Haijun DU
;
Mi LIU
;
Zhiqiang XIA
;
Jun HAN
;
Juan SONG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病预防控制国家重点实验室，北京 102206
- Keywords:
CVB3;
Viral pancreatitis;
Inflammatory infiltration;
Th17;
Treg;
Cytokines
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(2):128-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective:A mouse model of pancreatitis induced by coxsackievirus B3 (CVB3) was established. The pathological change of pancreas and the infiltration of Th17/Treg cells were observed.Methods:The BALB/c mice were inoculated intraperitoneally with CVB3 to induce acute viral pancreatitis model. Then the pathological changes of pancreas were observed by HE staining; the viral RNA load and relative expression of cytokines (IFN-γ, IL-6 and IL-17) mRNA were detected by q-PCR; the proportion of infiltrated CD45 + CD3 + T cells, CD4 + and CD8 + T cells, Th17 and Treg cells in the pancreas was determined by flow cytometry. Results:Three days after CVB3 infection, the viral RNA load in pancreas was the highest (0.96±0.18) and gradually decreased with prolongation of infection. Compared with the 3 dpi group, the viral RNA load in pancreas was decreased (0.96±0.18 vs. 0.62±0.14) at 7 dpi, but there was no statistically significant difference. In addition, the infiltration of immune cell in pancreas increased significantly after 7dpi and the pathological score >2. The percent of infiltrated Th17 cells (1.05±0.21 vs. 22.13±5.79) and Treg cells (3.11±0.78 vs. 8.25±1.30) among CD4 + T cells significantly increased after infection (P<0.05), and the Th17/Treg also increased (P<0.01). Compared with the control group, the relative mRNA expression of IFN-γ (1.05±0.23 vs. 672.6±47.67), IL-6 (1.00±0.38 vs. 68.28±4.57), and IL-17 (1.01±0.11 vs. 54.15±7.94) in pancreas increased at 7 days after CVB3 infection ( P<0.01). Conclusions:The infiltration of Th17/Treg cells and the expression of related cytokines related cytokines IL-6 and IL-17 mRNA were upregulated in pancreas, which promoted the process of CVB3-induced pancreatitis.
