Specific Killing Effect against Homo-leukemia Hybrid Cell Induced by a Multiple Epitope BCR-ABL Fusion Protein
10.3321/j.issn:1000-467X.2001.02.006
- VernacularTitle:BCR-ABL融合抗原诱导特异性同源白血病融合细胞杀伤效应研究
- Author:
Wei-Yang ZHENG
1
;
Xiao-Li LIU
;
He-Kui LAN
;
Fang YIN
;
Shu-Yun ZHOU
;
Ming-Hui ZHANG
;
Jian-Sheng HUANG
;
Da-Ming REN
Author Information
1. Nanfang Hospital the First Military Medical University
- From:Chinese Journal of Cancer
2001;20(2):144-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current study was designed to investigate the specific killing effects against homo-leukemia hybrid cell induced by BCR-ABL multiple epitope fusion protein and explore a powerful chronic myeloid leukemia(CML) immunotherapeutic strategy. Method： A multiple epitope antigen gene of BCR-ABL fusion antigen was used to immunize C57BL/6 mice and the spleen cells were cultured as effective cells. Specific lytic activities of target cells were detected by standard MTT assay. Results： Only the CTLs (cytotoxic lymphocytes) of mice immunized with the fusion protein can lyse the target cells but not the control cells (P<0.01 vs P>0.05). Conclusion： The multiple epitope BCR-ABL fusion antigen elicited antigen-specific, CTL-mediated anti-leukemia immune responses which provided a basis for further study on the developing specific T cell immunotherapy of CML in vivo.
