Variation of Cell Cycle and Telomerase Gene Expression in the Differentiation of Neuroblastoma Cells Induced by Retinoids
10.3321/j.issn:1000-467X.2001.02.004
- VernacularTitle:神经母细胞瘤细胞分化过程中细胞周期及端粒酶基因的变化
- Author:
Huan-Min WANG
1
;
Jin-Zhe ZHANG
;
Xun BI
;
Xiu-Dan ZHU
;
Jia-Ju LI
;
Hai-Yan ZHANG
;
Wei-Hong LI
Author Information
1. Affiliate Children's Hospital Capital Medical University of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(2):135-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The current study was designed to observe and discuss the variation of neuroblastoma cells in morphology, cell cycle and telomerase gene (hTR) expression induced by retinoids. Methods: Two newly-synthesized retinoids, R9158 and R8605, were supplemented into the media of neuroblastoma cells, SH-SY-5Y and SK-N-SH, with the concentration of 10- 6 mol/L as the treatment group. Meanwhile the same supplements of DMSO were as the control group. Pre-and post-supplement cells were observed for morphological changes, cell cycle was analyzed by flow cytometry (FCM) and hTR expression was analyzed by in situ hybridization chemistry (ISHH). Results: 1) The shape of SH-SY-5Y cells changed significantly at the 5th day of R9158 and R8605 treatment, having multiple neurite extensions just as mature neuron. However no shape change of SK-N-SH cells was found in observation for 7 days. 2)For SH-SY-5Y cells, the percentage of Stage G1, S, G2/M differed much between the treatment group and the control group at 5th day when the cell cycle of the treatment group was retarded at Stage G1 and the ratio of Stage decreased significantly. For SK-N-SH cells, however, there was little variation of cell cycle status in the treatment group. 3)The SH-SY-5Y cell numbers of hTR positive expression in the treatment groups decreased significantly at the 5th day. No decrease was found in the R9158 and R8605 groups of SK-N-SH cells in the treatment groups. Conclusions: During the differentiation of RA-sensitive cells induced by retinoids, the telomerase expression was depressed and the cell cycle retarded;whereas the RA-resistant cells changed little in morphology, telomerase expression and cell cycle as well. The telomerase gene associated with other genes would be an important regulated target in the differentiation induced by retinoids.
