Effect of HSP70 on Stability of Mutant p53 Protein
10.3321/j.issn:1000-467X.2001.02.003
- VernacularTitle:热休克蛋白70对突变型p53稳定性的影响
- Author:
Yun-Xia FAN
1
;
Mei ZHAO
;
Chang-Zhi HUANG
;
Can-Quan MAO
;
Chun ZHOU
;
Feng XU
;
Fei DU
;
Liang LIN
Author Information
1. Chinese Academy of Medical Sciences, Peking Union Medical College
- From:Chinese Journal of Cancer
2001;20(2):131-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current study was designed to investigate the effect of heat shock protein 70(HSP70) on stability of mutant p53 protein. Methods： Retroviral recombinant expressing antisense HSP70 RNA was constructed and transfected into MCF7/Adr breast cancer cells. The existence of foreign DNA was identified by PCR method and the HSP70 protein level was determined by Western blot analysis. The half-life of mutant p53 protein(mtp53) was measured by p53 stability assay. Results： The stable expressing strain(MAp70) from transfected cells was obtained through G418 selection. The foreign DNA in transfectant cells were confirmed by PCR, and the repression rate of HSP70 protein was 42％ . The half-life of mutant p53 in MAp70 cell was 12 hours, significantly lower than that of the control cells. Conclusion： Antisense HSP70 RNA can decrease the HSP70 protein level and significantly increase instability of mutant p53 protein in MCF7/Adr breast cancer cell.
