Establishment of a rabbit model of cardiopulmonary bypass in acute cerebral embolism phase
- VernacularTitle:兔脑栓塞急性期体外循环模型的建立及临床意义
- Author:
Wenkui MO
1
;
Liangyong HE
;
Qunqing CHEN
;
Yusheng YAN
;
Jian TONG
;
Hua MENG
;
Fuli ZHANG
Author Information
1. 南方医科大学珠江医院胸心外科
- Keywords:
cardiopulmonary bypass;
cerebral embolism;
rabbits;
models
- From:
Journal of Southern Medical University
2013;(11):1652-1655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a stable and feasible rabbit model of cardiopulmonary bypass (CPB) in acute cerebral embolism phase for studying the effects of CPB on brain tissues and the timing of surgical intervention of acute cerebral embolism. Methods Fifty-four rabbits were randomized into group A (n=18) to receive CPB without middle cerebral artery occlusion (MCAO) and group B to undergo CPB at 24 h (group B1, n=18) or 1 week (group B2, n=18) after MCAO. Through a supraorbital margin approach, electrocoagulation was carried out to occlude the main stem of the left MCA under direct vision to establish MCAO. Magnetic resonance imaging (MRI) was performed at both 24 h and 1 week after MCAO, and the severity of cerebral embolization was evaluated. CPB was established by cannulation of the ascending aorta and the right atrium through a median sternotomy incision. MRI was performed at 2 h after CPB to observe the brain tissues. Results MCAO was successfully established in groups B1 and B2, and all the rabbits survived after MCAO. In both groups A and B, MRI examination detected no cerebral hemorrhage or new embolism 2 h after CPB. Conclusions We have established a stable and feasible CPB model in rabbits with acute cerebral embolism to allow study of the mechanisms of CPB-related organ damage and its interventions.
