Senquence analysis of envelope gene of local dengue virus in Zhangzhou city, Fujian province, 2019
10.3760/cma.j.cn112866-20220425-00099
- VernacularTitle:2019年福建省漳州市本地流行登革病毒的E基因序列分析
- Author:
Liqing GUO
1
;
Meiyan XU
;
Qiuxia CHEN
;
Binbin YANG
;
Jinzhang WANG
Author Information
1. 漳州市疾病预防控制中心，漳州 363005
- Keywords:
Dengue fever;
Dengue virus;
E gene;
Phylogenetic tree
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the epidemiological characteristics and track probable imported sources of the local dengue outbreak in Zhangzhou city, Fujian province, 2019.Methods:Serum samples of patients with suspected dengue fever at acute phases were collected for virus detecting and serotyping by real-time fluorescence quantitative RT-PCR. For the positive specimens of local cases, full-length fragments of E gene were amplified by RT-PCR, and were sequenced and analyzed.Result:In 2019, there were 98 local cases of dengue fever in Zhangzhou city, which were concentrated in Zhao’an county, Longhai district and Yunxiao county. In this study, fourteen dengue virus E gene sequences representing different sources in different districts and counties were selected. The amino acid sequence virulence site analysis showed that the local epidemic strains were relatively virulent strains. The gene sequence alignment and phylogenetic analysis showed that all the local strains were classified as DENV-I subgenotype genotype I, divided into a, b and c three different branches. The evolutionary branch a contained all Zhao’an and Longhai sequences and was divided into three sub-branches, the b and c evolutionary branches were both the sequences of Yunxiao. There was a high correlation between the Shenqiao Town in Zhao’an and the Haicheng Town in Longhai. The other areas of the strains were limited to the towns, and the evolutionary branches were close to the other areas in China and countries in Southeast Asia.Conclusions:The indigenous dengue outbreaks in Zhangzhou, 2019 were caused by multiple sources of introduction and originated from other areas in China or from Southeast Asian countries and there was also the possibility of local cross-county transmission.
