Expression of p73 Gene in Lung Cancer Tissue
10.3321/j.issn:1000-467X.2001.01.012
- VernacularTitle:p73基因在肺癌组织中的表达
- Author:
Li HUANG
1
;
Yun-Jie WANG
;
Da-Xiang CUI
;
Kun LIU
;
Qing-Shu CHENG
Author Information
1. Tangdu Hospital the Forth Military Medical University
- From:Chinese Journal of Cancer
2001;20(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current study was designed to investigate the expression of p73 gene in lung cancer tissue. Methods： The authors detected quantitatively p73 gene expression in 40 specimens of lung cancer and matched paracancer tissue by RT PCR and analyzed the alteration of p73 by PCR and SSCP. Results： ① Using RT PCR, 24 specimens of lung cancer were determined to be overexpression of p73, 9 of which were moderately differentiated, 15 of which were poor differentiated; 9 of which were Stage Ⅰ-Ⅱ , 15 of which were Stage Ⅲ-Ⅳ . No specimen of paracancer tissue was screened out to be overexpression of p73. There was significantly different expression rates between lung cancer and paracancer tissue (P< 0.01). ② Allele specific analysis showed that expression of G/C： A/T appeared in 10 of 12 heterozygous lung cancer specimens and expression of G/C in 27 specimens. ③ No mutation was detected by SSCP. Conclusion： The expression of p73 gene may be related to differentiation level of lung cancer but not related to clinical stage.
