Detection and phylogenetic analysis of human rhinovirus in hospitalized patients with pneumonia in autumn and winter in Bengbu, Anhui province, 2021
10.3760/cma.j.cn112866-20220922-00198
- VernacularTitle:2021年安徽蚌埠秋冬季肺炎患者鼻病毒检测及基因特征分析
- Author:
Yanqing YANG
1
;
Yuanyou XU
;
Hongming DONG
;
Na LI
;
Ze WEI
;
Zhen GAO
;
Haijun DU
;
Guoyong MEI
;
Guoyu LU
;
Jun HAN
;
Chihong ZHAO
Author Information
1. 中国疾病预防控制中心，北京 102206
- Keywords:
Pneumonia;
Human rhinovirus;
Phylogenetic tree;
Coinfections
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(1):39-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate genetic characteristics of human rhinovirus (HRV) in adult inpatients with pneumonia in autumn and winter in Bengbu, Anhui province, 2021.Methods:The pharyngeal swabs of inpatients with pneumonia in Bengbu were collected for the detection of 14 common respiratory pathogens by Real-time PCR during September to December 2021. VP4/VP2 coding regions of HRV positive samples were amplified by nested PCR and phylogenetic tree was constructed using MEGA7.0.Results:A total of 146 samples were collected from inpatients with pneumonia; 35.62% (52/146) samples were positive with at least one pathogen. The four viruses with high detection rate were HRV, adenovirus, human coronavirus OC43 and influenza B virus. HRV positive samples accounted for 44.23% (23/52) of the positive samples, among which 9 cases (39.13%, 9/23) co-infected with HRV. Phylogenetic analysis found that HRV infection were dominated by HRV-A and HRV-B groups. The analysis based on clinical syndrome found that the white blood cells count and the proportion neutrophils of patients with HRV co-infection were higher that of HRV single infection. The proportion of patients with hypertension, diabetes, mechanical ventilation and poor prognosis in the HRV co-infection group were higher than that of HRV single infection group ( P<0.05). Conclusions:HRV is the predominant pathogen among the adult inpatients with pneumonia in Bengbu. HRV-A and HRV-B groups are common. Patients accompanied by hypertension, diabetes were easily co-infected with HRV. Patients coinfeted with HRV are more likely to be mechanical ventilation and poor prognosis.
