Nam Dinh virus was detected and isolated in arbovirus investigation in Shanxi, China
10.3760/cma.j.cn112866-20221212-00252
- VernacularTitle:首次在山西省虫媒病毒调查中检测并分离到南定病毒
- Author:
Shenghui LIU
1
;
Xiaodong TIAN
;
Weijia ZHANG
;
Hongmei ZHENG
;
Junying ZHAO
;
Chongxiao XU
;
Yali ZHANG
;
Shihong FU
;
Kai NIE
;
Fan LI
;
Peifang DAI
;
Qikai YIN
;
Ying HE
;
Jingxia CHENG
;
Huanyu WANG
Author Information
1. 山东第一医科大学（山东省医学科学院），济南 271016
- Keywords:
Arboviruses;
Nam Dinh virus;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(1):25-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In this study, the collected mosquito samples were subjected to viral isolation to identify the species and branch characteristics of arboviruses in five regions of Shanxi Province.Methods:Eight arboviruses in mosquito samples collected from July to September 2020 were detected by real-time fluorescent quantitative PCR, and virus isolation was carried out through cell culture. Virus isolates were identified and analyzed by molecular biology and bioinformatics method.Results:We detected 1 batch of positive samples of Japanese encephalitis virus, 2 batches of positive samples of Culex flavivirus and 8 batches of positive samples of Nam Dinh virus among 121 batches of mosquito samples. Seven virus isolates were isolated, numbered: SX-YJ-Cxp-4、SX-YJ-Ars-2、SX-YJ-Cxp-1、SX-LY-Cxp-10、SX-GP-Ars-5、SX-GP-Cxp-2、SX-GP-Cxp-4, all of which were identified as Nam Dinh virus, and the whole genome sequencing was performed on one of them, and the result showed that Shanxi Nam Dinh virus isolate and Yunnan Nam Dinh virus isolate belonged to the same evolutionary branch.Conclusions:Nam Dinh virus was isolated and identified on the specimen from Shanxi province for the first time.
