Immunological Reconstitution of T Cell Receptor Repertoire Diversity in Patients with Chronic Graft-versus-host Disease
10.3321/j.issn:1000-467X.2001.01.007
- VernacularTitle:慢性移植物抗宿主病患者T细胞受体Vβ亚家族T细胞的免疫重建
- Author:
Xin DU
1
;
Yang-Qiu LI
;
Wei LIN
;
Jian-Hua SUN
;
Zi-Lun HUANG
;
Shu-Nong LI
Author Information
1. Guangdong Province People's Hospital
- From:Chinese Journal of Cancer
2001;20(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current stady was designed to investigate the reconstitution of T cell receptor repertoire in the patients with chronic Graft-versus-host disease (cGVHD). Methods： The TCR repertoire in peripheral blood mononuclear cells from 5 cGVHD patients was examined after PCR amplification of 24 Vβ gene subfamilies. Results： Only 2－8 Vβ subfamily T cells were found in the samples from these patients, and there were different demonstrations in different patients. We found Vβ T cells proliferated in 4 patients. Conclusion： The TCR repertoire complexities was abnormal in all patients,Vβ may be associated with cGVHD, and the method may be demonstrated the reconstitution of T cell after transplantation.
