Polymorphisms of DNA Repair Gene XRCC1 in Chinese Population and its Relation to Esophageal Squamous Cell Carcinoma
10.3321/j.issn:1000-467X.2001.01.006
- VernacularTitle:中国人DNA修复基因XRCC1单核苷酸多态及其与食管癌风险的关系
- Author:
Chun-Ying SONG
1
;
Wen TAN
;
Dong-Xin LIN
Author Information
1. Chinese Academy of Medical Sciences and Peking Union Medical College
- From:Chinese Journal of Cancer
2001;20(1):28-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current study was designed to investigate the polymorphisms of DNA repair gene XRCC1 in Chinese population and test the hypothesis which these genetic variations may have impact on the risk of esophageal squamous cell carcinoma(ESCC). Methods： A case control study of 222 ESCC patients and 433 control subjects (matched for age, sex) was conducted to investigate the role of two XRCC1 polymorphisms(XRCC1 26304 T and XRCC1 281152 A) in ESCC. Genotyping was performed using PCR based restriction fragment length polymorphism techniques. The adjusted odds ratio (OR) and 95％ confidence interval (CI) was calculated using multivariate logistic regression. Results： Genotype frequencies of the XRCC1 C26304T were 51.7％ (CC), 41.6％ (CT) and 6.7％ (TT) in control group. The frequency of TT allele in control group (6.7％ ) was significantly lower than that in case group (12.6％ ), with the adjusted OR for ESCC being 1.83 (95％ CI 1.03－ 3.24; P< 0.05). Furthermore, the risk of ESCC for XRCC1 26304 TT alleles appeared to be more pronounced among smokers (adjusted OR=3.1,95％ CI 1.3－ 7.2) compared to nonsmokers (adjusted OR=2.3, 95％ CI 1.0－ 5.4), and among smokers who smoked≥ 20 cigarettes/day (adjusted OR=6.4, 95％ CI 2.0－ 23.7) compared to those who smoked< 20 cigarettes/day (adjusted OR=1.0, 95％ CI 0.2－ 4.3). Genotype frequencies of the XRCC1 G28152A were 53.3％ (GG), 38.1％ (GA) and 8.6％ (AA) in control group, which were not significantly different from that in case group. Conclusion： These findings support the hypothesis which polymorphism in XRCC1 DNA repair gene contributes to the risk of developing ESCC.
