Determination of Telomerase Activity and its Subunits in Different Gastric Lesions
10.3321/j.issn:1000-467X.2001.01.005
- VernacularTitle:不同病变胃粘膜端粒酶活性及其亚单位的检测
- Author:
Shi-Ming YANG
1
;
Dian-Chun FANG
;
Yuan-Hui LUO
;
Rong LU
;
Jin-Liang YANG
;
Wei-Wen LIU
Author Information
1. Southwest Hospital the Third Military Medical University
- From:Chinese Journal of Cancer
2001;20(1):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current study was designed to explore the role of telomerase and its subunits in the process of gastric carcinogenesis. Methods： Telomerase activity was detected with telomeric repeat amplification protocol (TRAP) and its subunits were examined by RT PCR. Results： Telomerase activity was noted positive in 24.6％ (14/57) for chronic atrophic gastritis, 38.9％ (7/18)for intestinal metaplasia, 37.5％ (3/8) for dysplasia and 92.3％ (62/65) for gastric carcinoma, respectively. On the contrary, no telomerase activity was detected in normal mucosa. The frequency of telomerase activity in gastric carcinoma was significantly higher than that in chronic atrophic gastritis, intestinal metaplasia and dysplasia (P< 0.01). Telomerase activity in gastric carcinoma was not related with clinicopathological parameters. Telomerase subunits, TP1 and hTR, were expressed in most gastric mucosas and no significant difference was found among different groups. However, another telomerase subunit,hTRT, was mainly detected in gastric carcinoma and partially premalignant lesions or diseases and the expression of hTRT paralleled to the expression of telomerase. Conclusions： Telomerase activitymay play an important role in the early stage of gastric carcinogenesis. hTRT may be not only a good diagnostic parameter but also a target for gene therapy in gastric cancer because hTRT expression parallels to telomerase activity.
