Cloning and Identification for Differentially Expressed cDNA in Nasopharyngeal Carcinoma Tissue
10.3321/j.issn:1000-467X.2001.01.004
- VernacularTitle:鼻咽癌组织差异表达cDNA序列的克隆与鉴定
- Author:
Ying-Hong MA
1
;
Xiu-Qing WANG
;
Mu-Sheng ZENG
;
Bing LI
;
Yi-Xin ZENG
Author Information
1. 中山医科大学附属肿瘤医院
- From:Chinese Journal of Cancer
2001;20(1):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective： The current study was designed to analyze the differentially expressed genes in nasopharyngeal carcinoma (NPC) tissue and identify the novel oncogenes and the tumor suppressor genes associated with NPC pathogenesis. Methods： Using the suppression subtractive hybridization (SSH), two SSH pools (normal nasopharyngeal tissue cDNA pool and NPC tissue cDNA pool) were constructed. Positive clones were screened by differential screen technique,and conformed using Southern blot and Northern blot, and sequenced with ABI 377 DNA sequencer. Results： Twelve positive clones were identified from SSH pools. Sequences of the 12 positive clones showed that 8 clones were homologous with the genes published in GenBank and 2 clones were unknown genes. The two novel genes NP5 and NP9 in the normal control cDNA pool were richer than that in the cancerous cDNA pool and NP9 was further found to be down regulated in both NPC cell lines and NPC tissue. Conclusions： The SSH is useful for analysis of the disease related genes and two novel cDNAs were identified to be related with NPC pathogenesis.
