Norcantharidin induces apoptosis in human breast cancer cell line
10.3321/j.issn:1000-467X.2000.12.003
- VernacularTitle:去甲斑蝥素对人乳腺癌细胞系的凋亡诱导作用及bcl-2基因的表达
- Author:
Yu RONG
1
;
Fu-You LIANG
;
Li CHEN
;
Hai-Jun DU
;
Le-Yao LIU
;
Hong-Liu SUN
;
Wei AN
Author Information
1. Capital University of Medical Sciences
- Keywords:
Norcantharidin;
Apoptosis;
Breast neoplasms
- From:Chinese Journal of Cancer
2000;19(12):1077-1081
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The current study was designed to identify molecular mechanism of antitumor effect produced by NCTD(norcantharidin) in human breast cancer cell line. Methods: MCF- 7 cells were grown in standard conditions. At the indicated time points after NCTD treatment, evaluation of apoptotic morphology by light microscopy, analysis of chromatin condensation by electron microscopy, determination of the percentage of apoptotic cells by flow cytometry and Western blot analysis for detecting of bcl- 2 gene expression were conducted. Results: Differences in cellular morphology were observed between NCTD- treated and control MCF- 7 cells. Cell shrinkage, cell budding and chromatin condensation were observed in the cells treated with 10 μ g/ml NCTD for 12 hours. Percentage of apoptotical cells was increased while the expression of bcl-2 products was decreased with the increasing of incubation time. Conclusion: The results indicate that one of the major antitumor mechanisms of NCTD may be through inducing cell apoptosis which related to the regulation of bcl- 2 gene expression.
