Differential gene expression profiling for identification of protective transcription factors in different subtypes of nasopharyngeal carcinoma
- VernacularTitle:基于差异性表达谱分析挖掘不同亚型鼻咽癌的保护性转录因子
- Author:
Chunyue HUANG
1
;
Pei LIN
;
Jiahong WANG
;
Zhongxi HUANG
Author Information
1. 南方医科大学1第一临床医学院
- Keywords:
nasopharyngeal carcinoma;
immune defense;
protective transcription factors;
survival rate
- From:
Journal of Southern Medical University
2013;(11):1565-1570
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the dysregulated genes among the differentially expressed genes in 41 nasopharyngeal biopsy samples and identify their protective transcriptional factors. Methods The differentially expressed gene profiles were obtained by analyzing both types I and II nasopharyngeal carcinoma (NPC_I and NPC_II, respectively) using EXcelland Bioinformatics tools. The transcriptional factors were further studied only when (1) the difference in the binding sites of the differentially expressed genes between NPC_I and NPC_II groups was statistically significant, (2) the expressions of the transcription factors were correlated with the gene expressions in the samples, and (3) the transcription factors affected at least 40%of the expression of the related genes. Results In NPC_I samples, 80 transcription factors were found to be up-regulated, in which RUNX3, GATA3, NR3C1, NRF1, RXRA, SMAD7, TBP, and ZBTB6 were positive factors and HLF and MTF1 were negative factors, involved in the regulation of the genes in T cell receptor signaling pathway. No eligible transcription factors were found in association with down-regulated genes in NPC_I compared to NPC_II gene expression profiles. Conclusions The over-expressed genes in NPC_I are mainly related to immune responses, and we found 8 positive factors and 2 negative factors that regulate the genes in T cell receptor signaling pathway. The 10 transcription factors may serve as potential therapeutic targets for NPC_I. We failed to identify any transcription factors associated with down-regulated genes in NPC_I relative to NPC_II possibly as a result of multiple factors that affect the differential gene expressions in NPC_II including the transcription factors, DNA phosphorylation and modification, chromosome variation and environmental factors.
