Study on the correlation between rotavirus infection and Histo-Blood Group Antigens in children with diarrhea in Nanjing
10.3760/cma.j.cn112866-20220519-00118
- VernacularTitle:南京腹泻患儿轮状病毒感染与受体组织血型抗原相关性研究
- Author:
Zhulun ZHUANG
1
;
Kunlong YAN
;
Yu JIN
;
Zhifeng LIU
;
Weixia CHENG
Author Information
1. 南京医科大学附属儿童医院，南京 210008
- Keywords:
Rotavirus;
Histo-blood group antigen;
Case-control study
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(6):691-695
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the prevalence of rotavirus infection and susceptibility to Histo-Blood Group Antigens (HBGAs) in children with diarrhea under 5 years of age in Nanjing.Methods:Stool and corresponding saliva samples were collected from 295 children with acute diarrhea and 150 healthy children. Rotaviruses were detected in stool samples by antigen detection method, and rotavirus positive samples were genotyped by RT-PCR method. HBGA phenotype of saliva samples was determined by anti-tissue blood group monoclonal antibody ELISA method. Rotavirus infection and HBGA susceptibility were analyzed by statistical analysis.Results:In the case group, 139 (47.12%) of 295 samples were rotavirus positive, with G9[P8] genotype being the most common genotype (84.17%, 117/139). The proportion of fever ( χ2=34.81, P<0.001), vomiting ( χ2=25.01, P<0.001) and respiratory symptoms ( χ2=4.73, P=0.03) in rotavirus infected children, which were higher than those in non-rotavirus infected group, and the difference was statistically significant. In the ABO blood group system, type AB children were more likely to have diarrhea (95% CI: 1.029~2.622; P= 0.036), and type B children had a higher risk of rotavirus infection ( OR=1.783, 95% CI: 1.027~3.095, P= 0.039). In the secretory system antigens, secretory children were more prone to diarrhea and rotavirus infection, G9[P8] genotype infection was related to secretory phenotype ( OR=2.854, 95% CI: 1.641~4.962), and non-secretory children ( χ2=5.723, P=0.017) were less susceptible to rotavirus and G9[P8] genotype rotavirus infection. Conclusions:G9[P8] genotype was the main rotavirus infection in diarrhea children under 5 years of age in Nanjing, and individuals with secretory phenotype were more likely to be infected with G9[P8], which provided scientific basis for preventing and controlling rotavirus diarrhea in this area.
