Expression of prion protein in papillary thyroid carcinoma and its relationship with clinical progression
10.3760/cma.j.cn112866-20220914-00194
- VernacularTitle:朊蛋白在乳头状甲状腺癌中的表达及与临床进展的关系
- Author:
Wei WEI
1
;
Xin LIU
;
Yuezhang WU
;
Yongping WANG
;
Qi SHI
Author Information
1. 北京大学肿瘤医院暨北京市肿瘤防治研究所头颈外科 恶性肿瘤发病机制及转化研究教育部重点实验室，北京 100142
- Keywords:
Prion;
Papillary thyroid cancer;
Immunohistochemistry;
Western blot
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(6):666-670
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the expression of prion protein in papillary thyroid carcinoma and its relationship with related clinical factors.Methods:Specimens from 60 patients with papillary thyroid cancer (PTC) diagnosed by pathology and 60 patients with benign thyroid lesions (BTL) were collected, and the prion proteins in the samples were detected and analyzed by immunohistochemistry, Western blotting and immunofluorescence. Statistical analysis was performed on the relationship between protein expression and patient-related clinical factors.Results:Immunohistochemistry showed a higher rate of prion positivity in papillary thyroid cancer tissue than in benign thyroid lesions ( χ2=36.310, P<0.001). Immunoblotting and immunofluorescence method show that prion protein expression is significantly higher in papillary thyroid cancer tissue than in patients with benign thyroid lesions[Immunoblot: (1.288±0.153)U/L vs. (0.667±0.036)U/L, t=6.855, P<0.01; Immunofluorescence: [Green fluorescence, 20X: (12.911±1.947)U/L vs. (36.322±3.797)U/L, t=9.502, P<0.001; Red fluorescence, 20X: (6.259±2.062)U/L vs. (28.229±2.611)U/L, t=11.437, P<0.001]. The expression of prion protein was significantly higher in clinical grade III papillary thyroid cancer tissues than in stage I and II patients, and all were significantly different (Stage I over III: χ2=6.301, P<0.05; Stage II over III: χ2=5.683, P<0.05). There was no correlation with the presence or absence of lymphatic metastases and no statistically significant correlation with the age or sex of the patients. Conclusions:Prion protein expression was increased in papillary thyroid carcinoma and correlated with clinical stage of the tumor. This study provides experimental evidence for prion protein as a molecular marker for targeted diagnosis and treatment of papillary thyroid cancer.
