2019 novel coronavirus antigen detection technology based on graphene field effect transistor
10.3760/cma.j.cn112866-20220330-00075
- VernacularTitle:基于石墨烯场效应晶体管的新型冠状病毒抗原检测技术
- Author:
Yuetong YANG
1
;
Changhao DAI
;
Dacheng WEI
Author Information
1. 复旦大学高分子科学系聚合物分子工程国家重点实验室，上海 200433
- Keywords:
2019 novel coronavirus;
Graphene field effect transistor;
Antigen
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(6):654-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to develop a promising transistor assay for 2019 novel coronavirus (2019-nCoV) antigen detection with easiness, high sensitivity and rapid response.Methods:A graphene field effect transistor (GFET) sensor with the channel material modified with antibody probes was fabricated. Transfer curves were measured at different 2019-nCoV spike protein concentrations to evaluate the validity and sensitivity of the method.Results:The antibody probes were successfully anchored on the graphene through linker molecules to ensure specificity and precision of this technique. Electrical measurements of the device showed a detectable concentration down to 3.6×10 -17 g/ml within a response time as short as 3 minutes. Conclusions:A sensitive, efficient and simple method for 2019-nCoV antigen detection through GFET was preliminarily established.
