Human stem cells from apical papilla can regenerate dentin-pulp complex
- VernacularTitle:人根尖乳头干细胞生成牙髓牙本质复合体的实验研究
- Author:
Huacui XIONG
1
;
Ke CHEN
;
Yibin HUANG
;
Caiqi LIU
Author Information
1. 广州市妇女儿童医疗中心口腔科
- Keywords:
dental papilla;
stem cells;
differentiation;
dentin-pulp complex
- From:
Journal of Southern Medical University
2013;(10):1512-1516
- CountryChina
- Language:Chinese
-
Abstract:
Objective To regenerate dentin-pulp complex by tissue engineering with human stem cells from apical papilla cells (SCAP) as the seed cells. Methods SCAP was separated from from normal human impacted third molars with immature roots by outgrowth culture. The cells were then cultured in the differentiation medium for 3 weeks or in normal medium for 60 days, and analyzed for mineralization potential by Alizarin red staining. The osteo/odontogenic markers including alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OC) and dentin sialoprotein (DSP) were investigated by immunofluorescence staining and reverse transcription-polymerase chain reaction. The co-cultured mixture of SCAP and HA/TCP, or HA/TCP alone was implanted subcutaneously on the back of nude mice for 8 weeks, and the implants were collected and examined by HE and immunohistochemical staining. Results Round alizarin red-positive nodules formed in the isolated cells after cell culture in the differentiation medium for 3 weeks or in normal medium for 60 days with positive staining for osteo/odontogenic markers. SCAP with HA/TCP could regenerate pulp-dentin complex-like tissue in nude mice. The cells near the dentin-like tissue were positive for DSP. No mineral tissue was found in mice receiving HA/TCP implantation. Conclusion SCAP may serve as a promising seed cell for dentin-pulp complex tissue engineering.
