Molecular characteristics analysis of imported dengue virus E gene in Sichuan province, China, 2012-2021
10.3760/cma.j.cn112866-20220407-00088
- VernacularTitle:四川省2012—2021年输入性登革病毒E基因分子特征分析
- Author:
Wei LI
1
;
Mingfeng JIANG
;
Hongrong ZHONG
;
Yiou CAO
;
Yuliang FENG
Author Information
1. 四川省疾病预防控制中心，成都 610041
- Keywords:
Dengue virus;
E gene;
Serotype;
Genotype;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(5):586-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the molecular characteristics of E genes of imported dengue virus (DENV) in Sichuan province from 2012 to 2021, such as serotypes and genotypes, and so to provide the basis for virus traceability.Methods:DENV E genes of the 28 serum samples which were tested DENV positive by real time RT-qPCR from 2012 to 2021 in Sichuan province were amplified and sequenced by RT-PCR. Bioinformatics software Mega Align5.0 was used for the alignment analyses of nucleotide and deduced amino acid sequences, and bioinformatics software Mega7.0 was used to draw phylogenetic trees.Results:RT-PCR and sequencing indicated that 28 E genes of DENV were obtained. The serotypes and genotypes of DENV were revealed by homology and phylogenetic analysis based on E genes of DENV; 20 virus strains of Sichuan belonged to DENV serotype 1 (DENV-1), of which 16 virus strains were genotype I (G-I), 4 virus strains were genotype V (G-V); 7 virus strains belonged to DENV serotype 2 (DENV-2), of which 4 virus strains were G-I, 3 virus strains were genotype II (G-II); 1 virus strain belonged to DENV serotype 3 (DENV-3), G-I. The phylogenetic trees showed that Sichuan strains had more homology with strains of Southeast Asia and Guangdong province in China, that is consistent with the epidemiological data.Conclusions:In 2012 to 2021, the imported DENV in Sichuan province had 3 serotypes and multiple genotypes, and they were most likely transmitted from Southeast Asian countries.
