Molecular epidemiological analysis of adenovirus in children with influenza-like illness in Xuzhou from 2013 to 2019
10.3760/cma.j.cn112866-20220307-00053
- VernacularTitle:2013—2019年徐州地区儿童流感样病例中腺病毒分子流行病学调查
- Author:
Wenwen ZHU
1
;
Weiwei WU
;
Xue GAO
;
Yanping XU
;
Wenting ZHANG
;
Yangguang DU
;
Jing TONG
Author Information
1. 徐州市疾病预防控制中心检验科，徐州 221006
- Keywords:
Adenoviruses;
Child;
Type detection;
Molecular epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(5):575-578
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the epidemiological characteristics of human adenovirus(HAdv)in Xuzhou area.Methods:A total of 7 257 swab samples from influenza-like illness children in Xuzhou children’s Hospital were collected during 2013-2019. Real-time PCR was used to detect adenovirus, and then the positive specimens were subjected to amplification for the hexon gene by polymerase chain reaction (PCR) for genotypic analysis. Adenovirus 55 was cultured by Hep-2 cells and analyzed by whole gene sequencing.Results:A total of 227 strains of adenoviruses were detected out of 7 257 samples, and the detection rate was 3.13%. Infections were mainly found in children aged 3 to 6, with a peak of detection in May and June. Typing data showed that the infection was mainly adenovirus type 3 and type 7, and the dominant type of adenovirus in different years alternated between type 3 and type 7. Complete gene sequencing showed only a few point mutations in genes of adenovirus type 55 we isolated, and no other gene mutation and recombination were found.Conclusions:The main types of adenovirus were types 3 and 7 among children with inluenza-like illness in Xuzhou from 2013 to 2019, and the infected population was mainly preschool children, and the detection peak was in spring and summer.
