Epidemiological characteristics and B3 hexon gene variation of human adenovirus in Wuxi from 2017 to 2020
10.3760/cma.j.cn112866-20220112-00004
- VernacularTitle:2017—2020年无锡市人腺病毒流行特征及B3亚型Hexon基因变异分析
- Author:
Guangyuan MA
1
;
Yong XIAO
;
Jing BAO
;
Hongxia GUAN
;
Qi ZHOU
;
Chun′an YU
Author Information
1. 南京医科大学附属无锡疾病预防控制中心，无锡 214023
- Keywords:
Human adenovirus;
Hexon gene;
Variation
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(5):569-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the genotyping, epidemic characteristics, genetic variation and main epidemic strains of human adenovirus (HAdV) in Wuxi city from 2017 to 2020.Methods:Real-time fluorescence PCR was used to detect the acute respiratory tract infection cases by testing the specimens submitted to sentinel hospitals from 2017 to 2020. The specimens of positive cases tested for nucleic acid were subjected to viral isolation via cell culture, Hexon gene sequencing and analysis were performed to determine genotype and variation.Results:The detection of human adenovirus was slightly different, there were two epidemic peaks in May to July and November to January in Wuxi from 2017 to 2020. The cases were mainly infants (1-3 years old), and the positive samples accounted for 32.05% (75/234). The main clinical symptoms were fever (65.81%), followed by cough (58.12%). There were 8 types of human adenovirus, belonging to 3 subgenera. HAdV-3 has become the main epidemic strain, accounting for 47.18% (67/142), the rest HAdV-7 13.38% (19/142), HAdV-55 7.75% (11/142), HAdV-1 9.15% (13/142), HAdV-2 9.15%(13/142), HAdV-5 5.63%(8/142), HAdV-6 2.11%(3/142), HAdV-4 5.63%(8/142). Compared with 9 domestic reference strains and international (KX384958.1-USA) reference strains, the local epidemic strain HAdV-3 has amino acid mutations at positions 623 and 938 in the evolutionary process.Conclusions:From 2017 to 2020, there are two epidemic peaks. The cases are mainly infants 1 to 3 years old. HAdV-3 has gradually become the dominant epidemic strain, and there are two high frequency mutation sites in the process of pressure selection and evolution.
