Comparison of chemical components between Baishao and Chishao water extracts and their effects on proliferation of rat thoracic aorta smooth muscle cells
- VernacularTitle:白芍、赤芍化学成分差异及其对大鼠胸动脉平滑肌A7r5细胞的生物效应比较
- Author:
Yaling ZHU
1
;
Chow YEUKLUNG
;
Jinxiong WU
;
Xian ZHANG
;
Liu YANG
Author Information
1. 广州中医药大学第二附属医院//广东省中医药科学院
- Keywords:
Paeonialactiflora;
Baishao;
Chishao;
A7r5 cells;
platelet-derived growth factor-BB
- From:
Journal of Southern Medical University
2013;(10):1453-1457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the chemical components in Baishao and Chishao water extracts and investigate their effects on the proliferation of rat thoracic aorta smooth muscle cells in vitro. Methods The contents and chemical structures of monomers separated from the water extracts of Baishao and Chishao were analyzed using high-performance liquid chromatography and mass spectroscopy. Rat thoracic aorta smooth muscle cell line A7r5 and its platelet-derived growth factor-BB (PDGF-BB)-induced proliferation model were exposed to different concentrations of Baishao and Chishao water extracts, and the cell viability was analyzed by mitochondrial-dependent reduction of MTT and real-time cell analyzer. Results The growth of A7r5 cells was significantly stimulated by 300μg/ml Baishao water extract (P<0.01), but Chishao water extract produced no such effect (P>0.05). In PDGF-BB-induced cell proliferation model, the cell growth was significantly suppressed by 100-500μg/ml Chishao water extract (P<0.01), while Baishao water extract showed no obvious effect on the cell proliferation (P>0.05). Conclusion Baishao and Chishao water extracts have different chemical components and produce different biological effects.
