Evaluation of different pathological staining methods for classifying renal amyloidosis
- VernacularTitle:区分肾淀粉样变类型的病理染色方法比较
- Author:
Zhanmei ZHOU
1
;
Wei CAO
Author Information
1. 南方医科大学南方医院肾脏病实验室
- Keywords:
kidney;
amyloidosis;
Congo red;
immunofluorescent staining;
immunohistochemistry
- From:
Journal of Southern Medical University
2013;(10):1448-1452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect of special staining and immunohistochemical staining in distinguishing the types of renal amyloidosis to improve the diagnosis accuracy. Method Congo red staining with different methods, and immunohistochemical staining of Kappa, Lambda and Amyloid A with different antigen retrieval methods were used for staining frozen and paraffin-embedded renal tissue sections. Results Wright's Congo red staining produced a better contrast and a higher resolution and showed a greater stability than the other 2 methods after repeated use for staining the renal tissue sections (P<0.05). Immunofluorescent staining produced better results in frozen renal tissue sections than in paraffin-embedded tissues. Immunofluorescent staining produced had better performance than immunohistochemical staining in paraffin-embedded tissues. The retrieval effect with protein kinase K was the best among the antigen retrieval methods in paraffin-embedded tissues. Conclusion Wright's Congo red staining is better than the other 2 methods in diagnosing renal amyloidosis. Immunohistochemical staining of Kappa, Lambda and Amyloid A in frozen renal tissue sections is necessary to discriminate the types of renal amyloidosis. For paraffin-embedded renal tissues, antigen retrieval using protein kinase K is better than the other 2 methods.
