Transcriptome characteristics of H1N1 influenza virus infected primary human retinal pigment epithelial cells
10.3760/cma.j.cn112866-20220621-00139
- VernacularTitle:H1N1流感病毒感染原代人视网膜色素上皮细胞的转录组学研究
- Author:
Hongli RAN
1
;
Jinmin TIAN
;
Yang HAN
;
Zhangfu CHEN
;
Yingze ZHAO
;
Yu LAN
;
J. William LIU
;
Xiangtian ZHOU
;
F. George GAO
Author Information
1. 温州医科大学附属眼视光医院，温州 325027
- Keywords:
H1N1 influenza virus;
Retinal pigment epithelial cell;
Transcriptome sequencing;
Differentially expressed genes
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(5):535-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Using high-throughput transcriptome sequencing technology to study the differentially expressed genes (DEGs) and related regulatory signaling pathways involved in H1N1 influenza virus infection in primary human retinal pigment epithelial (RPE) cells.Methods:Primary human RPE cells were infected with H1N1 influenza virus for 2 h or 12 h, respectively. Taking H1N1 uninfected cells as a control group, total RNA was extracted, a library was constructed, and transcriptome sequencing was performed. DEGs were screened by DESeq2 software, and DEGs were analyzed by gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway.Results:Compared with the control group, a total of 1830 DEGs were identified in the 2 h H1N1 influenza virus infection group, and 2847 DEGs were identified in the 12 h infection group; 1213 DEGs were identified in the H1N1 influenza virus infection kinetics process (2 h: 12 h). The GO functional annotation analysis of DEGs in the H1N1 influenza virus infection group for 12 h showed that DEGs widely exist in a variety of cellular components and participate in various biological processes such as cellular processes, biological regulation, and metabolic processes. KEGG pathway enrichment analysis showed that DEGs were mainly enriched in the PI3K-Akt signaling pathway, MAPK signaling pathway, cancer MicroRNAs, and cytokine-cytokine receptor interactions in the 2 h H1N1 influenza virus infection group; in the 12 h H1N1 influenza virus infection group, DEGs were mainly enriched in PI3K-Akt signaling pathway, cancer MicroRNAs, AGE-RAGE signaling pathway and immune-inflammatory pathways; during the kinetics of H1N1 influenza virus infection (2 h: 12 h), DEGs were mainly enriched in cytokine-cytokine receptor interaction, TGF-β signaling pathway.Conclusions:Infection with H1N1 influenza virus leads to significant changes in the transcriptome of RPE cells. These data provide scientific reference for elucidating the molecular mechanism of eye infection by respiratory viruses such as influenza virus.
