Epidemiological characteristics of norovirus GII infection in Tianjin city, 2017-2020
10.3760/cma.j.cn112866-20220228-00048
- VernacularTitle:2017—2020年天津市GII型诺如病毒感染的流行病学特征
- Author:
Yulian FANG
1
;
Jing WANG
;
Wei WANG
;
Mengzhu HOU
;
Lu WANG
;
Shuxiang LIN
;
Yu ZHAO
Author Information
1. 天津市儿童医院天津市儿科研究所 天津市儿童出生缺陷防治重点实验室，天津 300134
- Keywords:
Acute gastroenteritis;
Norovirus;
Genotype;
Epidemiology;
Children
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(5):521-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular epidemiologic and clinical characteristics of norovirus (NoV) in hospitalized children with acute gastroenteritis (AGE) in Tianjin to provide theoretical basis for clinical diagnosis and treatment.Methods:Fecal specimens were collected from children with AGE in Tianjin Children′s Hospital from August 2017 to July 2020. NoV was detected by real-time fluorescence reverse transcription-polymerase chain reaction (RT-PCR). Partial sequence of the capsid protein VP1 of positive samples was amplified by conventional RT-PCR. The products were sent for sequencing and genotyping based on the sequence. As the same time, the clinical symptoms were compared among children infected with different NoV genotypes.Results:Among the 6 432 specimens, 1 703 (26.5%) were positive for NoV. Sequence analysis showed that 761 identified NoV strains could be divided into 7 genotypes. Genotypes GII.4, GII.3, GII.2, GII.17, GII.1, GII.13 and GII.6 accounted for 55.5% (422/761), 36.7% (279/761), 4.9% (37/761), 0.9% (7/761), 0.8% (6/761), 0.8% (6/761) and 0.5% (4/761), respectively. All of the GII.4 belonged to GII.4 Sydney 2012. There was also a significant difference in the seasonal distribution of children infected with GII.4 and other genotypes ( χ2=103.53, P<0.001). Children infected with GII.4 NoV were more likely to have diarrhea, vomiting and dehydration ( χ2=8.42, P=0.004; χ2=20.39, P<0.001; χ2=4.99, P=0.025). Conclusions:GII.4 and GII.3 were mainly genotypes of NoV infection in Tianjin from 2017 to 2020 and genotypes were diverse. In addition, children with GII.4 NoV were more likely to suffer from diarrhea, vomiting and dehydration.
