Characteristics of norovirus in children with acute diarrhea in Beijing from 2014 to 2019
10.3760/cma.j.cn112866-20220722-00165
- VernacularTitle:2014—2019年北京市急性腹泻儿童中诺如病毒感染特征分析
- Author:
Liping JIA
1
;
Linqing ZHAO
;
Li DENG
;
Liying LIU
;
Huijin DONG
;
Hui HUANG
;
Fenghua JIN
;
Yuan QIAN
Author Information
1. 首都儿科研究所病毒学研究室 儿童病毒病病原学北京市重点实验室，北京 100020
- Keywords:
Children;
Norovirus;
Acute diarrhea;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(5):508-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the characters of norovirus infection in children with acute diarrhea in Beijing from 2014 to 2019.Methods:Fecal specimens were collected from children with acute diarrhea visiting the hospital affiliated to the Capital Institute of Pediatrics from April 2014 to December 2019 for norovirus screening by reverse transcription-polymerase chain reaction (RT-PCR). Then RdRp gene and VP1 gene of norovirus (NoV) were amplified and sequenced from positive specimens and then genotyped by Norovirus Genotyping Tool 2.0.Results:Fecal specimens were collected from 2 577 cases with acute diarrhea and tested for NoV RNA in this study, of which 484 cases (18.78%, 484/2 577) were positive for NoV. NoV infection can occur all year round, with obvious seasonal distribution, the epidemic peak is in autumn and winter. There was no gender difference in the detection of NoV ( χ2=1.77, P=0.183). 399 specimens genotyped by VP1 gene with 98.25% (392/399) being GII and GII.4 Sydney as the mainly genotype (52.13%, 208/399), followed by GII.3 (19.8%, 79/399) and GII.2 (18.5%, 74/399). Then 267 specimens also were genotyped by RdRp region, which revealed that the main genotype was GII.P31 (57.3 %, 153/267), followed by GII.P12 (15.36%, 41/267) and GII.P16 (14.98 %, 40/267). Dual-typing revealed that various combination strains, GII.4 Sydney[P31] (53.18%, 142/267) as the major combination, followed by GII.3[P12] and GII.2[P16] (15.36%, 41/267 and 10.49%, 28/267). While GII.3[P12] showed a downward trend, GII.4 Sydney[P16] increased gradually. Conclusions:Norovirus is an important pathogen of acute diarrhea in children in Beijing, from 2014 to 2019, with various prevalent genotypes.
