Genomic evolutionary analysis of norovirus GII.6P7 recombinant strain in China
10.3760/cma.j.cn112866-20220815-00179
- VernacularTitle:我国诺如病毒GII.6[P7]重组株基因组进化分析
- Author:
Xingyan WEI
1
;
Xi ZHU
;
Qing ZHANG
;
Xiangyu KONG
;
Mengxuan WANG
;
Yanhui YANG
;
Miao JIN
;
Zhaojun DUAN
Author Information
1. 国家卫生健康委员会医学病毒和病毒病重点实验室 中国疾病预防控制中心病毒病预防控制所，北京 100052
- Keywords:
Norovirus;
Acute gastroenteritis;
Outbreaks;
Genotype;
Evolution
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(5):501-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the evolutionary characteristics and key site variations of the GII.6[P7] genome of norovirus disease outbreak in China.Methods:Genome amplification and sequencing of 46 GII.6[P7] positive samples monitored from CaliciNet China from 2018 to 2021. Simultaneous integration of all ORF1 (GII. P7) and ORF2 (GII.6) sequences for Bayesian evolutionary analysis. And the use of Simplot for restructuring analysis.Results:According to Bayesian evolution analysis, GII. P7 polymerase has temporal evolutionary characteristics, with an average base replacement rate of 2.067× 10 -3 nucleotide substitution/site/year, and recombination with 4 different VP1 genotypes (GII.6, GII.7, GII.14, GII.20). In the capsid region, GII.6 noroviruses can be further divided into GII.6a, GII.6b and GII.6c subtypes. The 46 strains in this study belong to the GII.6a subtype, which are divided into the same cluster as the virus strain NHBGR59 circulating in China in 2015. Simplot analysis determined that the recombination site of the GII.6[P7] strain in this study was at the ORF1-2 junction. The amino acid site variation of VP1 mainly occurred at the end of P1.1 and the P2 region. Compared with the reference strain of GII.6a subtype, there was no variation in the receptor binding site. Conclusions:The GII.6[P7] recombinant strains of the norovirus outbreak from 2018 to 2021 in China all belong to the GII.6a[P7] subtype.
