Optimization of acoustic parameters of microbubble-enhanced ultrasound sonoporation for augmenting tumor cell permeability in vivo
10.3969/j.issn.1673-4254.2013.09.26
- VernacularTitle:超声致微泡声孔效应增强兔VX2肿瘤细胞通透性的适宜声学参数
- Author:
Lihong LUO
1
;
Fenglin WU
;
Baoping WANG
;
Tingting XU
;
Jie HU
;
Yang SUI
Author Information
1. 南方医科大学南方医院超声诊断科
- Keywords:
sonopration;
ultrasound;
microbubble;
permeability
- From:
Journal of Southern Medical University
2013;(9):1377-1381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the appropriate acoustic parameters for augmenting the permeability of tumor cells using microbubble-enhanced ultrasound. Methods Ninety intramuscular VX2 tumors grown in New Zealand white rabbits were randomly divided into 4 parts by 4 factors, namely sound intensity, pulse width, insonation time and microbubble dose, each at different levels. Therapeutic ultrasound was delivered directly to the tumor surface during intravenous infusion of microbubbles. The tumor was excised after the treatment and sliced to investigate the change in tumor cell permeability using lanthanum nitrate electron microscopy. The appropriate acoustic parameters of the 4 factors were investigated. Results Sonoporation induced by microbubble-enhanced ultrasound augmented the permeability of the tumor cells, and the appro-priate sound intensity, pulse width, insonation time and microbubble dose were 0.43 W/cm2, 2.42 ms, 10 min, and 0.50 ml/kg, respectively. Conclusion Sonoporation induced by microbubble-enhanced ultrasound can induce pores in tumor cell membrane, which may potential increase the efficacy of tumor chemotherapy.
