Surgical techniques and perioperative management for improving the success rate of orthotopic liver transplantation in rats
10.3969/j.issn.1673-4254.2013.09.23
- VernacularTitle:提高大鼠原位肝移植成功率的技术细节及围手术期处理
- Author:
Kebo ZHONG
1
;
Dinghua YANG
;
Xianghong LI
;
Liang LIN
;
Hua HE
;
Jiankun LIANG
;
Xiao LIU
;
Bo LIANG
;
Jialu LI
Author Information
1. 南方医科大学南方医院肝胆外科
- Keywords:
Orthotopic liver transplantation;
animal models;
anhepatic phase
- From:
Journal of Southern Medical University
2013;(9):1362-1366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the surgical techniques and appropriate perioperative management for ensuring successful orthotopic liver transplantation (ROLT) in rats. Methods Based on the double-cuff technique of Kamada, we modified the surgical techniques of separation, perfusion and cold preservation of the donor liver, shearing and anastomosis of the suprahepatic vena cava with optimized postoperative infusion protocols and animal care. Results Two hundred and senventy rats underwent ROLT and a learning curve of the success rate was built to reflect the impovment of techniques. The learning curve showed steep improvemts over the exploration stage, breakthrough stage and maturation stage, and the success rates incresed sharply over time (0%, 71.1%, and 94.5%, respectively) until finnally reaching over 90%. The shearing and anastomosis of the suprahepatic vena cava remained the most critical and difficult techniques in ROLT modeling. Conclusion Proficient microsurgical techniques and meticulous nursing can reduce postoperative complications, enhance operational success rate and extend the survival time after ROLT.
