Clinical application of metagenomic next-generation sequencing in pulmonary infection for patients with AIDS
10.3760/cma.j.cn112866-20210812-00146
- VernacularTitle:宏基因二代测序技术在艾滋病患者肺部感染中的临床应用
- Author:
Lin JIA
1
;
Ming CHEN
;
Yongfeng WU
;
Caiping GUO
;
Yulin ZHANG
;
Ronghua JIN
;
Yingmin MA
Author Information
1. 首都医科大学附属北京佑安医院感染与免疫医学科，北京 100069
- Keywords:
mNGS;
Diagnostic;
Pulmonary infection;
Immunodeficiency
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(4):441-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Comparing the diagnostic performance of pulmonary infection pathogens between metagenomic next-generation sequencing (mNGS) testing and traditional assay in patients with AIDS to provide references for the diagnosis and treatment of the disease.Methods:From July 2019 to January 2021, the regular clinical assays as well as mNGS testing were performed for patients and those discharged with a diagnosis of AIDS and pulmonary infection were retrospectively reviewed in the department of infectious diseases of Beijing You An Hospital. The cases with discharge diagnosis of AIDS for whom mNGS testing was performed on samples from respiratory system were analyzed. Diagnostic performance of pathogens was compared between mNGS testing and traditional etiologyic diagnostic method. Diagnosis concordance analysis and diagnostic comparison study between mNGS and traditional etiology diagnosis method in terms of pathogens were also performed.Results:Fifty-five cases discharged with AIDS and pulmonary infection were enrolled.. For 29 cases for whom mNGS testing was performed on samples from respiratory system, the sensitivity of mNGS for diagnosing infection was higher than that of traditional etiology diagnosis method (89.7% vs. 37.9%, P<0.001) but with poor consistency (Kappa=0.249, P=0.170). A superior positivity rate in mNGS than that in traditional etiology diagnosis method for diagnosing bacterial (90.9% vs. 9.1%, P<0.001) Pneumocystis jirovecii (mNGS only), and nontuberculous mycobacteria (mNGS only). Conclusions:mNGS could yield a higher sensitivity for pulmonary pathogen identification in AIDS patients, especially for bacterial, Pneumocystis jirovecii and nontuberculous mycobacteria compared to traditional etiologic diagnostic method.
