Establishment of a chronic obstructive pulmonary disease model by passive cigarette smoking and intratracheal LPS instillation in spontaneously hypertensive rats
10.3969/j.issn.1673-4254.2013.09.19
- VernacularTitle:烟熏联合脂多糖气管内滴注建立原发性高血压大鼠慢性肺阻塞模型
- Author:
Changhui YU
1
;
Yan CHEN
;
Ting LI
;
Wei LI
;
Shaoxi CAI
;
Ying MENG
Author Information
1. 南方医科大学南方医院呼吸内科
- Keywords:
chronic obstructive pulmonary disease;
animal models;
spontaneously hypertensive rat;
cigarette smoking
- From:
Journal of Southern Medical University
2013;(9):1341-1346
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a chronic obstructive pulmonary disease (COPD) model by passive cigarette smoking and (or) intratracheal instillation of lipopolysaccharide (LPS) in spontaneously hypertensive (SH) rats. Methods Fifteen male SH rats were randomly divided into control group, cigarette smoking exposure (CS) group and CS+LPS (cigarette smoking exposure plus intratracheal instillation of LPS) group. After 8 weeks' treatment, the COPD model was validated by inspecting the general condition and examining lung function and pulmonary pathological changes. The expressions of surfactant-associated protein A (SP-A), NF-κB, histone, p-Iκ-Kα/β, Iκ-Kα/β, and IκB-αwere determined with Western blotting, and the expression of TNF-αand IL-6 mRNA were measured using qRT-PCR. Results The rats in both CS and CS+LPS groups were marantic with intermittent cough and tachypnea. Lung function test showed increased RI and lowered peak expiratory flow in CS group, which were more prominent in CS+LPS group (P<0.05). HE staining demonstrated typical chronic bronchitic inflammation and emphysema in the lungs of the two model groups with significantly decreased mean alveolar number and significantly increased mean lining intermiment and destrction index. The emphysema level was more serious in CS+LPS group than in CS group. Western blotting showed markedly decreased expressions of SP-A and IκB-αin CS group and CS+LPS , especially the latter group. The protein levels of NF-κB, Iκ-K phosphorylation and mRNA expressions of TNF-α and IL-6 increased obviously in the two model groups. Conclusion COPD model can be established by passive smoking and (or) intratracheal instillation of LPS in SH rats, and the model induced by combined exposures is optimal.
