Approximate genome-wide evolutionary analysis of one human rotavirus G2P4 strain
10.3760/cma.j.cn112866-20220402-00082
- VernacularTitle:一株人轮状病毒G2P[4]型毒株的近似全基因组进化分析
- Author:
Shenghui GAO
1
;
Lili LI
;
Dandi LI
;
Xi ZHU
;
Mengxuan WANG
;
Mingwen WANG
;
Jianjun WU
;
Jinsong LI
Author Information
1. 甘肃中医药大学公共卫生学院，兰州 730099
- Keywords:
Rotavirus;
G2P[4] genotype;
Whole genome;
Phylogenetic tree
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(4):402-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the evolutionary characteristics of the approximate whole genome of rotavirus G2P[4] type 2020BJ strain.Methods:The rotavirus genome was amplified by reverse transcription-polymerase chain reaction (RT-PCR), the amplified products were sequenced, and the sequences were subjected to phylogenetic analysis and homology analysis.Results:The approximate full-length 11 segments of human rotavirus G2P[4] type 2020BJ strain were obtained. Sequence analysis showed that the strain was G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genotype (DS-1-Like); evolutionary analysis shows that it is closely related to strains in Japan, India, Bangladesh, Italy and other countries; there are differences in the amino acids of antigenic epitopes between the closely related strains.Conclusions:There are differences in the amino acids of the epitopes of VP7 and VP4 of the five G2P[4] rotavirus strains that are closely related to 2020BJ, which may lead to different epidemic characteristics, and rotavirus surveillance should be strengthened.
